Objective: To explore the expression level and clinical significance of teratocarcinoma-derived growth factor 1(TDGF-1, also called Cripto-1) in patients with renal clear cell carcinoma. Methods：Cripto-1 of mRNA and protein levels of 37 patients with renal clear cell carcinoma were measured by RT-PCR 、Western-blot and IHC and 37 paracancerous tissue served as control，further analysis of the relationship with the clinical pathologic characteristics Results：The Cripto-1 mRNA (0.620±0.069 vs 0.225±0.043，P=8.66*10^-7) and protein expression (0.895±0.360 vs 0.112±0.050，P=2.26*10^-8) in cancerous tissues  increased significantly as compared to the normal gastric tissues．Though there was no significant correlation between Cripto-1 expression with tumor pathological differential degrees. Consistently.IHC revealed that patients with lymph node metastasis(Z=-2.069, P=0.039) and those with more advanced clinical stages(F=0.015, P=0.032) were prone to show higher Cripto-1 protein level. Conclusion：Above results suggested that Cripto-1 was overexpressed in renal cancer tissues，which may contribute to cancerous progression and lymph node metastasis.