Abstract : Objective To establish non-muscle invasive bladder carcinoma xenograft model in nude mice . Methods  We injected the human bladder carcinoma cell line BIU-87 suspended in Matrigel into nude mice , recorded the incubation period and calculated the forming tumor percentage, measured long diameter , short diameter and weight of tumors, and then carry out the pathological identification of tumors. Results  All nude mice formed transplanted tumors. The average time of their formation was about 6-7 days. The average long diameter and short diameter of these tumors were about (19.58±1.85) mm and (14.18±1.59) mm, the average tumor volume were (2098.13±556.88) mm3 and the average tumor weight of (0.92±0.27) g. Pathological identification showed that cancer cells arranged is similar to transitional cell, but shows greater variability in cell size, shape, and greater loss of polarity. Conclusion  Applying Matrigel as a suspending agent successfully established superficial bladder cancer xenograft model in nude mice, and lay a foundation for further investigation on non-invasive bladder carcinoma.