Objective  To evaluate the clinical advantages of SonixGPS guided puncture positioning system and traditional ultrasound-guided percutaneous nephrolithotripsis (PCNL).Methods  A computer search was conducted on PubMed, EMbase, Cochrane Library, CNKI and Wanfang databases to collect literatures related to retrospective studies and randomized controlled trials (RCT) on the safety and effectiveness of SonixGPS-guided puncture compared with traditional ultrasound guidance in PCNL from the establishment of the database to December 2019. A Meta-analysis was performed by two participating researchers after independent literature screening, data extraction and evaluation of the risk of bias in the included studies.Results  A total of 7 studies were included, including 4 case-control studies and 3 RCT, with a total of 672 patients with upper urinary tract stones, including 334 patients in the SonixGPS guided group and 338 patients in the traditional ultrasound group. The results of Meta-analysis showed that compared with traditional ultrasound group, the operation time and puncture time in the SonixGPS guided group were shortened (MD=-15.31,95%CI: -18.71～-11.91, P<0.001) and the puncture time were shortened (MD=-2.03, 95%CI: -2.54～-1.52, P<0.001). In the SonixGPS guided group, the success rate of one puncture was higher (MD=6.79, 95%CI: 3.12～14.75, P<0.001), and the stone clearance rate was higher (MD=1.84, 95%CI:1.13～2.98, P=0.010), intraoperative blood loss decreased (MD=-0.58, 95%CI: 0.31～1.08, P=0.090).Conclusions  Compared with traditional ultrasound guidance, SonixGPS system has better operation time, puncture time, one puncture success rate, stone clearance rate and other aspects.