Objective: To investigate the effect and safety of minimally invasive percutaneous nephrolithotomy(MPCNL) with multiple tracts in a single session for branched renal calculi. Methods: Retrospectively analyzed the clinical data of   43 patients with branched  renal calculi 。Results:43 patients with branched renal calculi by multiple tracts in a single session in minimally invasive percutaneous  nephrolithotomy were analyzed. Results: All patients were successful in one-session access, in which 23 MPCNLs by two tracts, 12 by three tracts and 8 by four or more tracts. The single session stone-free rate was 84.8%（38/43）. The mean operative time was （126±45.7）minutes. During and after operation, 4 cases needed transfusion and one patient underwent selective embolization of renal artery. No septic shock occurred. Conclusion: Minimally invasive percutaneous nephrolithotomy with multiple tracts in a single session was a effective and safe treatment method for the patients with branched renal calculi.