Objective To investigate efficiency and safety of indocyanine green (ICG) fluorescence imaging technique in robotassisted laparoscopic partial nephrectomy for complicated renal tumors.Methods Between June 2021 and December 2021, clinical data of 22 patients with complicated renal tumors underwent Da Vinci robot-assisted partial nephrectomy under ICG fluorescence real-time navigation at the first affiliated hospital of Fujian medical university by a single physician. ICG was injected 0.25 mg/kg through the median elbow vein 2 min before blocking renal artery. The ICG fluorescence real-time navigation was used for identifying the edge of tumor. The basic information and perioperative data of all patients were collected and analyzed statistically.Results A total of 16 males and 6 females were included, with a median age of 53 (34~72) years and a body mass index of 24.8 (19.8~26.2) kg/m2. There were 10 cases (45%) of tumors in the upper pole of the kidney, 7 cases (32%) in the middle of the kidney, and 5 cases (23%) in the lower pole of the kidney. The tumor diameter was 6.3 (3.4~9.4) cm. The preoperative clinical stages were T1aN0M0 in 2 cases (9%), TlbN0M0 in 18 cases (82%) and T2aN0M0 in 2 cases (9%). The preoperative R.E.N.A.L.score was 8 (7~11). All the 22 patients were successfully operated, and no patients were transferred to open, no severe allergic symptoms occurred in patients with intraoperative ICG injection, and no positive results were found in all surgical margins. The median operation time was 119 (102~235) min and the median renal artery occlusion heat ischemia time was 20 (18~29) min. The median intraoperative blood loss was about 60 (40~300) mL without transfusion. The median length of postoperative hospitalization was 6 (4~9) days. There were no serious complications and no tumor recurrence and metastasis during postoperative follow-up.Conclusions The application of near-infrared IGC fluorescence imaging technology under the guidance of Da Vinci Xi robot has emerged as an effective and safe tool that may facilitate partial nephrectomy for complicated renal tumors.