Abstract：Objective To detect the expression of DEADbox helicase 5 (DDX5) and microRNA-205 (miR-205) in prostate cancer (PCa), and to analyze their relationship with the prognosis of PCa.Methods A total of 86 patients with PCa who underwent radical prostatectomy or needle biopsy in shuangqiao hospital of chaoyang district from April 2015 to April 2017 were selected as the research objects. The cancer tissues and adjacent tissues were taken as PCa group and control group respectively. Real-time fluorescent quantitative PCR (qRT-PCR) was used to detect the levels of DDX5 mRNA and miR-205 in the tissues of the subjects. Immunohistochemical method was used to detect the expression of DDX5. The relationship between the expression of DDX5, miR-205 and clinicopathological features was analyzed. Kaplan-Meier survival analysis was used to analyze the relationship between the expression levels of DDX5 and miR-205 and the prognosis of patients with PCa. Cox regression analysis was used to analyze the influencing factors of poor prognosis of PCa.Results The expression levels of DDX5 mRNA and protein in PCa group were higher than those in control group (all P<0.05), and the expression levels of miR-205 were lower than those in control group (P<0.05). The expressions of DDX5 and miR-205 in PCa patients were correlated with tumor stage, serum PSA, Gleason score and tumor metastasis (all P<0.05), but not with age and nature of resection margin (all P>0.05). Kaplan-meier survival analysis showed that the 3year cumulative survival rate of DDX5positive patients was lower than that of DDX5negative patients (P<0.05). The 3year cumulative survival rate of patients with high miR-205 expression was higher than that of patients with low miR205 expression (P<0.01). Multivariate Cox analysis showed that higher DDX5 level and lower miR-205 level were risk factors for poor prognosis of PCa (HR=2.598, 3.342, all P<0.01).Conclusions DDX5 was highly expressed in the cancer tissues of PCa patients, while miR-205 was low expressed, which were associated with various clinicopathological and prognosis of PCa, and were risk factors for poor prognosis of PCa patients.
