Abstract：To investigate the effect of G protein coupled receptor 75 (GPR75) receptor mediated 20-hydroxyeicosatetraenoic acid (20-HETE) signaling pathway on proliferation, migration and invasion of androgen independent prostate cancer cells.Methods PC-3 androgen-independent prostate cancer cell lines were cultured in vitro, and divided into four groups, control group (no treatment), shGPR75 group (transfection of knockdown GPR75 receptor), 20-HETE group (20-HETE intervention) and shGPR75+20-HETE group (transfection of knockdown GPR75 receptor+20-HETE intervention). Then various indexes were detected using different methods, including expression of GPR75 by Western blot, proliferation of PC-3 cells by Methyl thiazolyl tetrazolium (MTT) assay, invasion ability of PC-3 cells by Transwell chamber assay, and migration ability of PC-3 cells by scratch test.Results The expression level of GPR75 protein was (1.04±0.13) in control group, (0.42±0.03) in shGPR75 group, (1.27±1.20) in 20-HETE group, and (0.68±0.07) in shGPR75+20-HETE group, with statistical difference (P<0.05). The survival rate of PC-3 cell was (76.30±10.30) in control group, (40.33±4.60) in shGPR75 group, (88.50±12.61) in 20-HETE group, and (54.38±6.70) in shGPR75+20-HETE group, with statistical difference (P<0.05). Transwell chamber assay showed that the invasion ability of PC-3 cells was (107.30±15.39) in control group, (62.30±7.20) in shGPR75 group, (123.57±18.90) in 20-HETE group, and (80.11±10.61) in shGPR75+20-HETE group, with statistical difference (P<0.05). The migration ability of PC-3 cells was (142.50±20.33) in control group, (86.24±10.60) in shGPR75 group, (170.93±24.78) in 20-HETE group, and (113.65±16.01) in shGPR75+20-HETE group, with statistical difference (P<0.05). Conclusions 20-HETE can promote the proliferation, invasion and migration of androgen non-replacement prostate cancer cells, which may be related to the reduction of the cancer-promoting effect of 20-HETE by inhibiting the GPR75 receptor.
