Objective To investigate the change of double hydrogen testosterone(DHT) and flutamide(FLU) on the on the mitochondrion in prostate cancer cells LNCaP.Methods  Drogen-dependent(LNCaP) prostate cancer cell lines and cell lines with stable growth and transmission were acquired by cell culture.  the androgen receptor agonist DHT and the androgen receptor   antagonist FLU applied to the culture prostate cancer cell line of androgen at different concentrations drugs.Mitochondria at different time points of LNCaP under the interventions were extracted. Mitochondrial radidal and differences at different time points and under the action of different drug concentration in LNCaP were measured and compared.Results  After DHT acted on LNCaP cells, the GSH of mitochondron decreased significantly（P﹤0.05）, but the difference of the decreasing degree of each index in different time points was found. After FLU acted on LNCaP cells, the content of GSH increased significantlyon the Middle dose group（P﹤0.05）,the other groups was not significantly（P>0.05）.Conclusions  DHT can cause decreasing of GSH on the mitochondrion,and promote cell proliferation.FLU can not remove the GSH on LNCaP cells,and cause the accumulation of free radicals, can inhibit the proliferation of LNCaP cells effectively.